In order to further investigate the correlation between clinical parameters and the combined expression of B7-H3 and B7-H4 in the esophageal cancer tissues, the 103 patients were categorized into two subgroups according to the staining intensity (H-score) of B7-H3 (cut-off value = 125), and B7-H4 (cut-off value = 160). This evidence concerns the gene CD276 and esophageal cancer.