Western blot analysis indicated different levels of ZNF703 protein that expressed not only in normal human intrahepatic biliary cell line but all the five CCA cell lines, in which QBC939 and TFK-1 cell lines as highly aggressive CCA cells expressed more ZNF703 while RBE and HuCCT1 cells with lower capability of invasion and metastasis expressed nearly the same levels of ZNF703, compared to the control normal HIBEpiC cell. Here, ZNF703 is linked to cholangiocarcinoma.