H2BC21 and infection: When PLA and IFA were performed to assess the IFI16-H2B complex at various times of infection, we observed increased association of IFI16 and H2B in the cytoplasm of infected cells from 2 to 12 h p.i. which was reduced at 24 h p.i. (Fig 2H, yellow arrows and S2D and S2E Fig).