These results demonstrated that IFI16 and H2B, and H2B and BRCA1 associate in the nucleus of uninfected and KSHV infected cells which redistribute to the cytoplasm only after infection and suggest that the presence of nuclear KSHV DNA is necessary for the H2B-IFI16 and H2B-BRCA1 cytoplasmic localization. This evidence concerns the gene IFI16 and infection.