Since we observed that H2B associates with IFI16 in the nucleus of uninfected and KSHV infected cells (Figs 1 and 2) and H2B was also pulled down by KSHV and HSV-1 genome during de novo infection (Fig 9A and 9B), we next determined whether H2B participates in viral genome recognition by IFI16. Here, H2BC21 is linked to infection.