Similarly, in PLA reactions to detect the EdU-labeled HSV-1 genome association with IFI16 during de novo HFF cell infection we observed substantial levels of EdU-HSV-1 and IFI16-IFI16 association in the nucleus (Fig 9E, top right panel, white arrows and Fig 9F) which was reduced significantly in siH2B infected cells (Fig 9E, middle right panel, white arrows and Fig 9F). This evidence concerns the gene IFI16 and infection.