Our analysis demonstrates that Tsg101 interacts with both upstream complex Hrs and with downstream complex proteins, EAP45 (ESCRT-II), CHMP6 (ESCRT-III) and CHMP5 (ESCRT-III), and CHMP5 and CHMP6 proteins associate with KSHV (glycoprotein gB) particles early during infection (S2 and S3 Figs). This evidence concerns the gene TSG101 and infection.