TSG101 and infection: To determine whether Tsg101 plays any roles in the KSHV entry and/or nuclear delivery of viral genome stages of infection [15], siControl and siTsg101 transfected HMVEC-d and HUVEC cells were infected with KSHV for 2 h, washed, treated with 0.25% trypsin-EDTA for 5 min at 37°C to remove the bound but noninternalized virus, washed, treated with DNAse I for 10 min at 37°C, and real-time DNA PCR carried out with extracted DNA.