In this study, we performed Kaplan-Meier survival analyses and log-rank tests to evaluate the association between RACK1 expression levels and survival outcomes of glioma patients for the first time, discovering that patients with high RACK1 expression had notably shorter overall survival time than those with low RACK1 expression, which was compatible with the research results by Cao et al. and Li et al. that up-regulated expression of RACK1 promoted tumor growth and predicted poor prognosis in breast cancer and pancreatic ductal adenocarcinoma patients [26,31]. This evidence concerns the gene RACK1 and glioma.