The Treg transcription factor FOXP3 was significantly lower (p < 0.01), while CD25 (IL2RA) was significantly higher (p < 0.0001) in PDAC (n = 147) than in skin cutaneous melanoma (n = 472) indicating that CD25 is expressed in PDAC by non-regulatory T cells as well (Supplementary Fig. 3). This evidence concerns the gene FOXP3 and cutaneous melanoma.