The expression of TNF-α and P65 (NF-κB) were enhanced to about 3.0 fold and 2.66 fold respectively at transcript level (Fig 3A), while about 3.0 fold and 2.0 fold enhanced respectively at protein level (Fig 3B and 3C) in DEN-induced hepatic cancer as compared to control mice. This evidence concerns the gene RELA and liver cancer.