MDA-MB-231 cells cultured in presence of MG and treated with TGF-β (2.5 ng/ml) responded by a two-fold increase of CTGF mRNA level compared to TGF-β alone confirming the requirement of TGF-β pathway activation for MG-mediated induction of CTGF expression (Figure 5C). The gene discussed is CCN2; the disease is myasthenia gravis.