Specifically, we injected B16-F1 melanoma murine cells ectopically expressing the p53 W146*, R213*, G325* mutants, as well as the vector control Td-Tomato (Figure 2—figure supplement 2), into C57BL/6J mice via tail vein as previously described (Overwijk and Restifo, 2001). The gene discussed is TP53; the disease is melanoma.