It was difficult to evaluate the biological effect of C5L2 for gastric cancer cells, because siRNA used for C5aR knockdown also downregulated the C5L2 expression (Supplementary Figure S2B), It was suggested that C5L2 does not associated with cancer cell invasiveness via RhoA because C5L2 does not couple to G protein [12, 39, 40]. This evidence concerns the gene C5AR1 and cancer.