The isolation of ctx-positive V. cholerae O1 in a non-cholera outbreak period from fish samples in Tanzania is of food safety and public health concern [8–16].The presence of the toxin co-regulated pilus (tcp-A) is important as this gene encodes for fimbriae synthesis which allows the bacteria to adhere to the host’s intestinal epithelium [17]. This evidence concerns the gene CYP27A1 and vibrio infectious disease.