After EA treatment, the protein expression of PGP9.5 was significantly increased compared to constipation models in jejunum (Fig. 3a, b, c), ileum (Fig. 3e, f, g) and proximal colon (Fig. 3i, j, k), but there was no difference compared with the control group, respectively (Fig. 3d, h, l). This evidence concerns the gene UCHL1 and Constipation.