We subsequently pretreated the cells with the EGFR specific inhibitor AG1478 at 5 μM prior to SC19 infection, and we observed that the tyrosine phosphorylation of these identified proteins decreased markedly after AG1478 pretreatment, suggesting the possible involvement of EGFR and its related signaling molecules in SS2 strain infection of hBMEC. This evidence concerns the gene EGFR and infection.