The results using staining with CNPase (Figure 2e–h) and MOBP resembled that with MBP, since statistical analysis revealed a significant reduction in signals in TSC and FCD IIB compared to the control (Kruskal–Wallis, pairwise comparisons: CNPase: P = 0.001*, P = 0.039*; data not shown MOBP: P = 0.003*, P = 0.148 n.s.). This evidence concerns the gene MBP and tuberous sclerosis.