In order to know if Notch-dependent regulation of PlexinD1 widely occurs in cancer cells, we stably knocked-down Notch1 expression by shRNA in a range of cell lines derived from colon (KM-20, COLO741), prostate (PC3), lung cancer (A549) and melanoma (MDA435), and consistently observed PlexinD1 downregulation (S3A Fig). This evidence concerns the gene NOTCH1 and melanoma.