TMA techniques (collective analysis) and conventional histological slides (individual analysis) showed fair agreement when they evaluated the immunoreactivity of PD-L1 expression in tumor cells (Kappa = 0.307) and in antigen presenting cells (Kappa = 0.328) of patients with non-small-cell lung cancer, p value < 0.001, proving that TMA is not an adequate method to evaluate PD-L1 expression. Here, CD274 is linked to non-small cell lung carcinoma.