The feasibility of this process was demonstrated for the bovine endothelial Per-ARNT-Sim (PAS) domain-containing protein 1 gene (EPAS1) encoding the hypoxia inducible transcription factor 2A (HIF2A).EPAS1 was selected for analysis because two linked missense mutations were reported to be associated with pulmonary hypertension (PH) in Angus cattle15. The gene discussed is EPAS1; the disease is pulmonary hypertension.