INS and Hyperglycemia: However, previous studies have demonstrated that subclinical β-cell defects could be identified even in insulin-sensitive nondiabetic offspring of patients by using hyperglycemia-hyperinsulinemic clamp, the OGTT, or the IVGTT [23, 24, 29], but decline of HOMA-B was not observed during early stage of abnormal glucose tolerance, leading to underestimate the magnitude of the -β-cell defect [31].