EIF4E and infection: Substitution of both Asn-160 and Gln-161 into Asp and Lys (N160D and Q161K), respectively, abolished KoA eIF4E activity to facilitate JK05 infection in rym5 (cultivar Misato Golden) protoplasts, while substitution of Thr-120 into Ser (T120S) only reduced virus accumulation (Figure 4E).