To identify the effect of SENP1 on glycolysis in ccRCC, the mRNA expression levels of the key glycolytic enzymes, including PGK1, PFK1, ENO1, ALDOA, CA9, LDHA, and HK2, in ccRCC tumor and adjacent normal tissues were examined by real-time RT-PCR. This evidence concerns the gene HK2 and nonpapillary renal cell carcinoma.