To know the role of 7-KC in the pathogenesis of cardiovascular diseases, the purposes of this study were to investigate the effect of 7-KC on the growth, Ataxia Telangiectasia Mutated Protein (ATM)/Ataxia telangiectasia and Rad3 related (ATR)-cell cycle checkpoint kinase-1 (Chk1)/Chk2, p53, cell cycle kinetics, apoptosis and IL-8 production of endothelial cells. The gene discussed is CALCA; the disease is cardiovascular disorder.