Interestingly, we found that PAK inhibition or depletion in PAK1 amplified or overexpressing breast cancer cells significantly reduced the formation of Rad51 foci, but had no effect in PAK1 non-amplified breast cancer cells which were able to form Rad51 foci in response to DNA damaging agents (Figure 2A and 2B). This evidence concerns the gene RAD51 and breast cancer.