By using ROC analyses for classification of severe fibrosis in NAFLD patients, we obtained the cutoff values of serum YKL-40 (165 ng/mL), type IV collagen 7s (6.1 ng/ml), hyaluronic acid (46 ng/ml), WFA+ -M2BP (1.12 COI), APRI (0.89), and FIB-4 index (1.6) (Table 3, Supplementary Figure S3). This evidence concerns the gene CHI3L1 and metabolic dysfunction-associated steatotic liver disease.