As drug-induced cell death is dependent on the tumor suppressor protein p53 in hepatoma cells [24], we next assessed the effectiveness of Amitriptyline respect to a standard genotoxic treatment with the topoisomerase I inhibitor camptothecin (CPT) in three hepatoma cell lines with different p53 status: HepG2 with wild-type p53, Huh 7 with mutated p53 and Hep3B with non-sense mutation on p53 gene. Here, TCHP is linked to hepatocellular carcinoma.