We then assessed protein expression of ASS1 by immunoblotting in cancer cell lines derived from osteosarcoma (U-2 OS, MNNG/HOS, MG-63, NOS-1 and HuO 9N2), leiomyosarcoma (SK-LMS-1, SK-UT-1 and SK-UT-1B), synovial sarcoma (SYO-1 and Fuji), chondrosarcoma (HCH-1), Ewing's sarcoma (LUPI, RD-ES and SK-ES) and alveolar soft part sarcoma (ASPS-1). This evidence concerns the gene ASS1 and osteosarcoma.