RT-PCR analysis revealed that the ZNF216 mRNA was variably expressed among the cell lines (Figure 1A) with the highest level of expression found in melanoma cell line MDA-MB-435 weakly expressing EGFR and the lowest levels shown in breast carcinoma cell lines T-47D and HBL-100 moderately expressing EGFR mRNA [32] A431 cell line showed the highest level of EGFR gene expression, paralleled by a very low expression of ZNF216 mRNA. This evidence concerns the gene ZFAND5 and melanoma.