When analyzed by FACS for a senescent-like phenotype (i.e. elevated size and/or AF) (Supplementary Fig. S3)30, we observed significantly higher number of cells bearing senescent-like features in BUB1 and SMC1A-depleted cultures relative to EV (shB2: p = 0.0013; shS2: p < 0.0001), similar to what observed in SEN cultures (SEN: p < 0.0001) (Fig. 4b). The gene discussed is SMC1A; the disease is atrial fibrillation.