Based on our pre-experimental, the relatively low concentrations of thapsigargin (0.6 and 0.3 μM) and TRAIL (70 and 35 ng/ml) did not affect the cell viability and phosphorylation of AMPK in human ESCC cells (Supplementary Figure 1A,B). Here, TNFSF10 is linked to esophageal squamous cell carcinoma.