Indeed, the cells with the strongest ability to form tumorspheres were those infected and transfected with siIQGAP1, implying that the inhibition of IQGAP1 promotes tumorsphere formation in response to H. pylori infection, especially in AGS and MKN-74 cells where the association of the transfection and the infection led to 40 and 20 times more tumorspheres, respectively. Here, IQGAP1 is linked to infection.