Moreover, our results show that after the treatment of solanine in DU145 cells, the expression level of phosphorylated P38/ATF2 is significantly upregulated, which can be blocked by the administration of P38 inhibitor SB203580 and the active oxygen scavenger NAC, indicating the P38 pathway is downstream of ROS induction in solanine‐induced apoptosis and tumor inhibition. This evidence concerns the gene ATF2 and neoplasm.