TBC1D32 and Lyme disease: We compared our best mChip-Ld candidate (rP100+PepVF+OspC-K) to the C6 ELISA which is generally used as a first tier assay for serodiagnosis of Lyme disease (Fig. 5, Supplementary Table 1) and we found that the sensitivity for overall diagnosis of Lyme is just as high for microfluidic test as for the C6 ELISA test.