CXCL16 and neoplasm: We next evaluated the levels of secreted CXCL16 using an enzyme-linked immunosorbent assay (ELISA) on supernatants from primary CAFs and showed that CAFs isolated from TN tumours (3/4) secreted high amounts of CXCL16, while low CXCL16 was measured in CAFs isolated from ER+ tumours (0/8) (Fig. 6c).