Under these conditions, inflammatory cytokine (TNF-α), inflammatory marker protein (serum amyloid A (SAA)), macrophage markers (F4/80 and CD11c) and monocyte chemotactic protein (MCP)-1 mRNA expression levels were greater in mice fed the 0.1% and 0.2% methionine in HFCD diets than in those fed the control and HF diets, and these changes were attenuated by increasing the methionine content. The gene discussed is CCL2; the disease is hydrops fetalis.