Recent studies demonstrated that a high-fat and high-cholesterol (60% energy from fat and 1.25% cholesterol) diet containing sodium cholate induced NASH in mice, and the pathological features of this model were similar to those of humans, such as steatohepatitis, inflammation, fibrosis [17], lipid metabolism, insulin resistance, and hepatic gene expression profiles responsible for liver pathology in a mouse model [18]. This evidence concerns the gene INS and metabolic dysfunction-associated steatohepatitis.