To examine the interaction between PARP1 and DUX4 promoter in human myoblasts, we performed a ChIP assay targeting the promoter region containing a putative PARP1 binding site located at 112-116 bp upstream of the transcription start site [41] using immortalized human myoblasts from an individual with FSHD and myoblasts from the subject's unaffected sibling as control [37]. This evidence concerns the gene DUX4 and facioscapulohumeral muscular dystrophy.