Based on the in silico kinetic modeling analysis indicating that both uncompetitive and mixed-type inhibitors can perturb at a significantly greater extent the pathway flux and metabolite concentrations than competitive inhibitors, it is concluded that elevation of the Fru1,6BP levels will have a more severe depressing effect on the glycolytic flux of cancer cells than that of DHAP levels because the former behaves as a mixed-type inhibitor of HK whereas the latter competitively inhibits HPI (Moreno-Sánchez et al., 2016). The gene discussed is HK1; the disease is cancer.