Given the massive Dies1 downregulation in GC cell lines, and in order to identify a potential mechanism that could downregulate Dies1 in the two GC cell lines (MKN45 and MKN28) lacking promoter methylation, we explored the expression of miR-125a-5p, known to target Dies1 3′-UTR in mouse ESCs31, in these two GC cell lines. The gene discussed is VSIR; the disease is gastric cancer.