Again, SHH-treated EB cells expressed high levels of FOXG1, SOX1, LHX6, and LHX8, while SHH-treated AD cells expressed high levels of FOXA2, but almost complete lack of FOXG1, SOX1, LHX6, or LHX8 expression (Figures S2A–S2H). Here, LHX8 is linked to Alzheimer disease.