CHL1 and Alzheimer disease: Moreover, SHH-patterned AD cells expressed much higher levels of EN1, WNT5A, RAX, and CHL1, representative marker genes for midbrain and ventral diencephalon (Liu et al., 2000, Parr et al., 1993, Shimogori et al., 2010), at an expense of FOXG1 expression as compared to SHH-treated EB cells (Figure 2F).