For example, the reference DHS re13.110396155 (located ~10 kb upstream of the prostate cancer associated IRS2 gene [36, 37]) presents as a DHS in PrEC but not in LNCaP, and accordingly, WGBS data show the region to be lowly methylated in PrEC and highly methylated in LNCaP. This evidence concerns the gene IRS2 and Familial prostate cancer.