ISG15 and parasitic infectious disease: C57BL/6 mice infected with N67 parasite induced a strong IFN-I response, including increased expression of genes such as Cd40, Isg15, Isg20, Ifit2, Mx1, Mx2, Ddx58, and Usp18 genes (S1A and S1B Fig), leading to suppression of N67 parasitemia [8, 38].