To determine which MNT to test in a mouse model of ALS, we assessed the effects of 3 MNTs, BNN20, BNN27, and BNN23 (at 1, 10 and 30 μM), in human astrocytes (iAstrocytes) derived from ALS patients with SOD1 mutations co-cultured with mouse motor neurons (MNs) in vitro. The gene discussed is SOD1; the disease is amyotrophic lateral sclerosis.