Since BNN27 (10 μM) yielded the greatest effect on MN survival, we further tested its neuroprotective effects in iAstrocytes derived from two individual ALS patients harboring different SOD1 mutations (SOD1 91 and SOD1 210) (Table 1) along with Riluzole (10 μM), a commonly used ALS therapeutic. This evidence concerns the gene SOD1 and amyotrophic lateral sclerosis.