HP and Schnyder corneal dystrophy: Fig 3A, 3C and 3E shows the ApoA-1 level was decreased in plasma and HDL of SCD mice. However, cf-Hb released by sickled red cells showed the opposite result (Fig 3B and 3D). We also observed significant increases in Hb, Hp and Hx in ApoA-1 particles of HDL in SCD mice (Fig 3F). Based on linear regression and the data above, it appears that ApoA-I is necessary for the combination between HDL and Hb/Hp/Hx complexes. Furthermore, we demonstrated by the immuoprecipitation and immunoblot methods that the amount of Hb associated with HDL was much higher than in the controls (Fig 4A).