Fig 3A, 3C and 3E shows the ApoA-1 level was decreased in plasma and HDL of SCD mice. However, cf-Hb released by sickled red cells showed the opposite result (Fig 3B and 3D). We also observed significant increases in Hb, Hp and Hx in ApoA-1 particles of HDL in SCD mice (Fig 3F). Based on linear regression and the data above, it appears that ApoA-I is necessary for the combination between HDL and Hb/Hp/Hx complexes. Furthermore, we demonstrated by the immuoprecipitation and immunoblot methods that the amount of Hb associated with HDL was much higher than in the controls (Fig 4A). This evidence concerns the gene APOA1 and Schnyder corneal dystrophy.