We also demonstrated that depleting both glucose and glutamine in DLBCL cells or treating cells with an HBP inhibitor (azaserine) diminished O-GlcNAc protein substrate levels, inhibited constitutive NF-κB and NFATc1 activation, and induced G0/G1 cell-cycle arrest and apoptosis. This evidence concerns the gene NFKB1 and diffuse large B-cell lymphoma.