To correlate the degree of cytokine inhibition by NI-0101 in individual RASF-induced cytokine production assays, with ACPA and TLR4 ligand levels in RASF, a sample was designated as non-responder or responder to NI-0101 blockade based on the ability of NI-0101 to decrease RASF-induced IL-6 production from RA monocytes (Fig. 2). The gene discussed is TLR4; the disease is rheumatoid arthritis.