In this study, we established a rat model of focal cerebral ischemia/reperfusion and found that activated astrocytes and microglia act as inflammatory cells in brain tissue and that the accumulation of pro-inflammatory cytokines such as TNF-α and IL-1β contribute to the formation of cerebral infarction, deteriorated neurological function, and the induction of inflammatory brain damage. Here, TNF is linked to Cerebral ischemia.