To definitely explore the notion that FASN-driven endogenous lipogenesis might constitute an attractive therapeutic target for eliminating CCN1-overexpressing breast cancer cells, we employed a flow cytometric fluorescence-based method to discriminate damaged/dead from viable cells in immunofluorescently labeled populations using propidium iodide (PI) as a dyeexclusion viability probe. Here, CCN1 is linked to breast cancer.