ERBB2 and neoplasm: Cases with a diffuse intense circumferential membrane staining pattern in > 30% of the tumor (scored as 3+) should be considered positive by IHC, and when using FISH, HER2 positivity is defined as a HER2 gene copy > 6 or a HER2/CEP17 ratio > 2.2 (Hicks & Schiffhauer, 2011; Vranic et al., 2011).