As shown in Table 1, media conditioned by premalignant lesion cells increased spleen cell production of the Th1 mediators IL-2 (p = 0.0002) and IFN-γ (p < 0.0001), the inflammatory mediators TNFα (p < 0.0001), IL-6 (p < 0.0001), and IL-17A (p < 0.0001), and the inhibitory mediator IL-10 (p < 0.0001) compared to media conditioned by HNSCC cells or media alone. Here, TNF is linked to head and neck squamous cell carcinoma.