Furthermore, Compound 1 could not promote cell proliferation in RhoA silenced MDA-MB-231 and MCF7 cells compared with cells transfected with siCtrl alone, indicating that RhoA is able to reverse the inhibitory effect on cell proliferation by NRF2 downregulation in breast cancer cells (Figure 6D–6J). The gene discussed is NFE2L2; the disease is breast carcinoma.