To determine the potential cytotoxic effect of CFZ on NB cells, the CCK-8 assay was measured on six NB cell lines, including three N-myc amplified cell lines (IMR-32, NB-19, NGP) and three N-myc non-amplified cell lines (LA-N-6, SH-SY5Y, SK-N-AS) (Figure 1). The gene discussed is MYCN; the disease is neuroblastoma.