The incubation of HCC cells with a neutral sphingomyelinase 2 (nSMase) inhibitor GW4869 resulted in an unchanged cellular expression and reduced exosomal expression of miR-16, indicating that the release of specific miRNAs from HCC cells into exosomes might occur via a ceramide dependent manner [28, 35]. Here, SMPD2 is linked to hepatocellular carcinoma.